rabbit anti glut4 polyclonal ab  (Novus Biologicals)

Journal: Molecular metabolism

Article Title: Pancreatic beta-cell specific BAG3 knockout results in chronic hyperinsulinemia inducing insulin resistance.

doi: 10.1016/j.molmet.2023.101752

Figure Lengend Snippet: Figure 5: BAG3bKO mice displayed peripheral but not hepatic insulin resistance. (A) Venn diagram and volcano plot showing the number of proteins and DEPs detected in muscles of 64-weeks old control and BAG3bKO mice. Down-regulated and up-regulated DEPs identified by MS/MS analysis are highlighted in blue and red, respectively. (B) Significantly inhibited upstream predicted by IPA analysis. (C, D) Representative Western blot of muscle INSR, GLUT4 phospho-AKT/AKT, and phospho-ERK1/2/ERK1/2 expression in control and BAG3bKO mice. Relative protein expression data are presented as mean values SEM; n ¼ 7e8 for each group; *p < 0.05.

Article Snippet: The membranes were incubated overnight at 4 C with primary antibodies: anti-BAG3 used 1:2000 (Novus Biologicals, Milan, Italy), anti-INSR used 1:1000, anti-AKT used 1:1000, anti-phospho-AKT used 1:1000, anti-ERK1/2 used 1:1000, antiphospho-ERK1/2 and anti-GAPDH used 1:2000, all supplied by Cell Signaling Technology (Danvers, MA, USA), anti-GLUT4 Rabbit polyclonal Ab used 1:1000 (Antibodies.com, Cat. #95872), anti p62/ SQSTM1 used 1:1000 (BD Bioscience, San Jose, CA, USA), anti-Insulin B (C-12) used 1:500 (Santa Cruz Biotechnology) and anti-b-actin used 1:40,000 (SigmaeAldrich, Milan, Italy).

Techniques: Muscles, Control, Tandem Mass Spectroscopy, Western Blot, Expressing